David Kleinfeld Laboratory at UCSD
 

LabTech Links

Solutions/Surgery

Surgical Guide for Rat Microdrive
Urethane Useage Guide
Artificial Cerebral Spinal Fluid
Preparation of 4 % Paraformadehyde
Nissl Staining


Extracellular Recording

Headstage Buffer
Differential Preamplifier
Highpass Filter Bank
Lowpass Filter Bank
Fullwave Rectification for EMG
National Instruments DAQ Board


In vivo Imaging

Headframe
Hamamatsu PMT
PMT Amplifier
Intermediate Amplifier
DAC Voltage Reference
Integrator
Stepping Motor Controller
Slow Current to Voltage Converter
Cambridge Scanners
Cambridge Drivers
National Instruments ADC Board
National Instruments DAC Board


Guides on Femtosecond Laser Microscopy

Principles, design and construction of a two photon scanning microscope for in vitro and in vivo studies. P. S. Tsai, N. Nishimura, E. J. Yoder, A. White, E. Dolnick and D. Kleinfeld. In Methods for In Vivo Optical Imaging (R. Frostig, editor), 2002, CRC Press, pp. 113-171.
PDF (2,453kB)

All-optical, in situ histology of neuronal tissue with femtosecond laser pulses. P. S. Tsai, B. Friedman, C. B. Schaffer, J. A. Squier and D. Kleinfeld. In Imaging in Neuroscience and Development: A Laboratory Manual (R. Yuste and A. Konnerth, editors), 2005, Cold Spring Harbor Laboratory Press, NY, 102:815-826  PDF (1,607kB)

Two-photon imaging of neocortical microcirculation. D. Kleinfeld and W. Denk. In Imaging Neurons: A Laboratory Manual (R. Yuste, F. Lanni, and A. Konnerth, editors), 1999, Cold Spring Harbor Laboratory Press, NY, pp. 23.1-23.15.   PDF (1,261kB)

 


Guides on Data Analysis

Spectral analysis for dynamical imaging data. B. Pesarian, A. Sornborger, N. Nishimura, D. Kleinfeld and P.P. Mitra. In Imaging in Neuroscience and Development: A Laboratory Manual (R. Yuste and A. Konnerth, editors), 2005, Cold Spring Harbor Laboratory Press, NY, 57:439-444.   PDF (1,175kB)

 

 

David Kleinfeld, PhD
Physics Department
UC San Diego
dk@physics.ucsd.edu